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Objective To explore the changes of bone metabolism in children with newly diagnosed primary nephrotic syndrome (PNS). Methods Forty-five children diagnosed with PNS from February 2013 to July 2016 were selected into the PNS group, and 30 healthy children in the same period were enrolled in the control group. The serum calcium, phosphorus, bone alkaline phosphatase (BALP), osteocalcin (BGP), type I collagen cross linked C terminal peptide (CTx) and serum tartrate resistant acid phosphatase 5b (TRACP), and 25-(OH)D were measured and compared between two groups. Bone mineral density (BMD) was measured by ultrasound. Results The levels of BALP, CTx, and 25-(OH)D in the PNS group were higher than those in the control group, and the levels of BGP, TRACP, blood calcium, and BMD (Z- scores) in the PNS group were lower than those in the control group, and the differences were statistically significant (P<0.01). There was no significant difference in serum phosphorus between two groups (P>0.05). Conclusions Abnormal bone metabolism is found in children with newly diagnosed PNS and its manifestations include calcium loss, reduced bone formation, and increased bone resorption.
ABSTRACT
Objective To explore the changes of bone metabolism in children with newly diagnosed primary nephrotic syndrome (PNS). Methods Forty-five children diagnosed with PNS from February 2013 to July 2016 were selected into the PNS group, and 30 healthy children in the same period were enrolled in the control group. The serum calcium, phosphorus, bone alkaline phosphatase (BALP), osteocalcin (BGP), type I collagen cross linked C terminal peptide (CTx) and serum tartrate resistant acid phosphatase 5b (TRACP), and 25-(OH)D were measured and compared between two groups. Bone mineral density (BMD) was measured by ultrasound. Results The levels of BALP, CTx, and 25-(OH)D in the PNS group were higher than those in the control group, and the levels of BGP, TRACP, blood calcium, and BMD (Z- scores) in the PNS group were lower than those in the control group, and the differences were statistically significant (P<0.01). There was no significant difference in serum phosphorus between two groups (P>0.05). Conclusions Abnormal bone metabolism is found in children with newly diagnosed PNS and its manifestations include calcium loss, reduced bone formation, and increased bone resorption.
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Objective To explore the expression of resistin-like molecules-a (RELMa) in atherosclerotic plaque of ApoE-/- mouse, and to study the effects of RELMa on the proliferation and migration of vascular smooth musclee cells (VSMCs) . Method Nine ApoE-/- mice and nine C57BL/6J mice were fed with high fat diet. All mice were sacrificed 24 weeks after force feeding. Vessels were dissected from to abdominal aorta. Sections of aortic tissue were stained with HE dyeing and RELMa in aortic tissue was assayed by immunohistochemistry. The expression of RELMa mRNA in vessels was detected by RT-PCR. The effects of different concentration RELMa in different concentrations on the proliferation and migration of VSMCs were detected. Data was expressed as mean ± standard deviation. ANOVA were used for comparison in SPSS 11.0, and changes were considered as statistically significant if P value was less than 0.05. Results Atherosclerosis plaque formed in aortic root of ApoE-/- mice after they were fed with high fat diet for 24 weeks. RELMa protein and RELMa mRNA were found by immunohistochemistry and RT-PCR in atherosclerotic plaque of ApoE-/- mouse. RELMa protein didn't be found in vessels of control mouse. RELMa promoted the proliferation of VSMCs (RELMa groups: 2811. 21 ± 216. 89,4056. 87 ±220.65,5061.45 ± 335.86, vs. control 1609.58 ± 203.53, P < 0.01). RELMa promoted the migration of VSMCs (RELMa groups: 130.54±12.98,158.39±11.58,203.50± 17.37 vs. control:70.54± 11.92, P<0.01).Conclusions RELMa expresses in atherosclerotic plaque of ApoE-/- mouse. RELMa enhances the proliferation and migration of VSMCs of aorta.
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Objective To investigate the effects of atorvastatin on cardiovascular remodeling in renovascular hypertensive rats. Methods The 2-kindey,1-clip hypertensive rats(2K1C,Goldblatt) were prepared with Sprague-Dawley rat.SD rats were randomly divided into three groups: normal control rats,hypertensive rats and hypertensive rats treated with atorvastatin(2 mg?kg~(-1)?d~(-1)).After 6 weeks treatment,systolic blood pressure(SBP) was measured using the tail-cuff method.The plasma concentration of angiotensin Ⅱ and renin activity were determined by radioimmunoassay.The heart weight,the ratio of left ventricular weight to body weight were calculated.Results The plasma concentration of angiotensin Ⅱ((106.4?7.8)ng/L) and renin activity((20.6?2.4) ng/L)were significantly increaed in hypertensive rats compared with normal rats((72.3?5.4) ng/L and(12.5?3.7) ng/L)(P
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Objective To study the effects of Laurencia terpenoids(LET)on the levels of IL-6 and TNF-?in H_(22) mice.Methods 1.The content of total terpenoids in LET was detected by RP-HPLC.2.The mice were randomly divided into five groups:the model group,the LET groups(25,50,100mg?kg~(-1)?d~(-1)) and the Cyclophosphamide(CY) group,and transplant tumor model of hepatoma 22(H_(22)) was established.The next day four groups of animal(except the model group) were treated with different dosage of LET and CY(ig) and executed after 15 days.The tumors were weighed and the tumor inhibition rates were calculated.Furthermore the levels of IL-6 and TNF-?in serum were determined by RIA.Results In vivo, the increases of H_(22) tumor weight in LET groups were slower than that in model group.The tumor inhibition rates were 27.5%,34.9%and 41.4%correspondently.Mid and high-dose groups of LET could increase the levels of IL-6 and TNF-?.Conclusion LET could inhibit the increase of H_(22) tumor weight.The mechanism may be related to enhancing the level of cytokine including IL-6 and TNF-?.